Broad specificity microbial enzymes

Broad specificity microbial enzymes: An annotated selection of World Wide Web sites relevant to the topics in microbial biotechnology

Review on enzyme promiscuity and opportunities for engineering http://www.weizmann.ac.il/Biological_Chemistry/scientist/ Tawfik/papers/(109)BarEven_CoOpBiot_2013.pdf This review article discusses a number of enzymes with broad specificity in the context of enzyme promiscuity. Soluble methane monooxygenase: KEGG database http://www.genome.jp/dbget-bin/ www_bget?ec:1.14.13.25 Soluble methane mono...

A Measure of the Broad Substrate Specificity of Enzymes Based on ‘Duplicate’ Catalytic Residues

The ability of an enzyme to select and act upon a specific class of compounds with unerring precision and efficiency is an essential feature of life. Simultaneously, these enzymes often catalyze the reaction of a range of similar substrates of the same class, and also have promiscuous activities on unrelated substrates. Previously, we have established a methodology to quantify promiscuous activ...

Post-polyketide synthase steps in iso-migrastatin biosynthesis, featuring tailoring enzymes with broad substrate specificity.

The iso-migrastatin (iso-MGS) biosynthetic gene cluster from Streptomyces platensis NRRL 18993 consists of 11 genes, featuring an acyltransferase (AT)-less type I polyketide synthase (PKS) and three tailoring enzymes MgsIJK. Systematic inactivation of mgsIJK in S. platensis enabled us to (i) identify two nascent products of the iso-MGS AT-less type I PKS, establishing an unprecedented novel fea...

Monoclonal Antibodies with Broad Specificity

Immobilization of microbial enzymes

Improvement of properties by immobilization http://www.scitopics.com/IMPROVEMENT_OF_ ENZYME_PROPERTIES_VIA_IMMOBILIZATION_ TECHNIQUES.html This webpage focuses on the means by which enzyme activity can be stabilized or improved in an immobilized state compared with the same enzyme free in aqueous solution. Immobilized enzymes http://www.rpi.edu/dept/chem-eng/Biotech-Environ/viet/ nelly.html Thi...